Part:BBa_K644001:Design

Candida albicans hyphal wall protein 1 (HWP1) gene

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 617
  Illegal BamHI site found at 1
  Illegal BamHI site found at 1053
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Since there is an EcoRI restriction endonuclease site on the HWP1 gene sequence, we had to use the Gibson Assembly method to clone the HWP1 gene into the BioBrick plasmid pSB1C3.

Source

Candida albicans